40S ribosomal protein S27a is a protein that in humans is encoded by the RPS27Agene.[1][2]
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi.[2]
References
↑Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (Aug 1998). "A map of 75 human ribosomal protein genes". Genome Res. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID9582194.
Pancré V, Pierce RJ, Fournier F, et al. (1991). "Effect of ubiquitin on platelet functions: possible identity with platelet activity suppressive lymphokine (PASL)". Eur. J. Immunol. 21 (11): 2735–41. doi:10.1002/eji.1830211113. PMID1657614.
Kanayama H, Tanaka K, Aki M, et al. (1992). "Changes in expressions of proteasome and ubiquitin genes in human renal cancer cells". Cancer Res. 51 (24): 6677–85. PMID1660345.
Monia BP, Ecker DJ, Jonnalagadda S, et al. (1989). "Gene synthesis, expression, and processing of human ubiquitin carboxyl extension proteins". J. Biol. Chem. 264 (7): 4093–103. PMID2537304.
Redman KL, Rechsteiner M (1989). "Identification of the long ubiquitin extension as ribosomal protein S27a". Nature. 338 (6214): 438–40. doi:10.1038/338438a0. PMID2538756.
Lund PK, Moats-Staats BM, Simmons JG, et al. (1985). "Nucleotide sequence analysis of a cDNA encoding human ubiquitin reveals that ubiquitin is synthesized as a precursor". J. Biol. Chem. 260 (12): 7609–13. PMID2581967.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
Vladimirov SN, Ivanov AV, Karpova GG, et al. (1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry". Eur. J. Biochem. 239 (1): 144–9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID8706699.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Kirschner LS, Stratakis CA (2000). "Structure of the human ubiquitin fusion gene Uba80 (RPS27a) and one of its pseudogenes". Biochem. Biophys. Res. Commun. 270 (3): 1106–10. doi:10.1006/bbrc.2000.2568. PMID10772958.
Bolton D, Evans PA, Stott K, Broadhurst RW (2002). "Structure and properties of a dimeric N-terminal fragment of human ubiquitin". J. Mol. Biol. 314 (4): 773–87. doi:10.1006/jmbi.2001.5181. PMID11733996.
Cohen BD, Bariteau JT, Magenis LM, Dias JA (2003). "Regulation of follitropin receptor cell surface residency by the ubiquitin-proteasome pathway". Endocrinology. 144 (10): 4393–402. doi:10.1210/en.2002-0063. PMID12960054.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID14702039.
Li H, Seth A (2004). "An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein". Oncogene. 23 (10): 1801–8. doi:10.1038/sj.onc.1207319. PMID14755250.
1aar: STRUCTURE OF A DIUBIQUITIN CONJUGATE AND A MODEL FOR INTERACTION WITH UBIQUITIN CONJUGATING ENZYME (E2)
PDB 1cmx EBI.jpg
1cmx: STRUCTURAL BASIS FOR THE SPECIFICITY OF UBIQUITIN C-TERMINAL HYDROLASES
PDB 1d3z EBI.jpg
1d3z: UBIQUITIN NMR STRUCTURE
PDB 1f9j EBI.jpg
1f9j: STRUCTURE OF A NEW CRYSTAL FORM OF TETRAUBIQUITIN
PDB 1fxt EBI.jpg
1fxt: STRUCTURE OF A CONJUGATING ENZYME-UBIQUITIN THIOLESTER COMPLEX
PDB 1g6j EBI.jpg
1g6j: STRUCTURE OF RECOMBINANT HUMAN UBIQUITIN IN AOT REVERSE MICELLES
PDB 1gjz EBI.jpg
1gjz: SOLUTION STRUCTURE OF A DIMERIC N-TERMINAL FRAGMENT OF HUMAN UBIQUITIN
PDB 1nbf EBI.jpg
1nbf: Crystal structure of a UBP-family deubiquitinating enzyme in isolation and in complex with ubiquitin aldehyde
PDB 1ogw EBI.jpg
1ogw: SYNTHETIC UBIQUITIN WITH FLUORO-LEU AT 50 AND 67
PDB 1otr EBI.jpg
1otr: Solution Structure of a CUE-Ubiquitin Complex
PDB 1p3q EBI.jpg
1p3q: Mechanism of Ubiquitin Recognition by the CUE Domain of VPS9
PDB 1q0w EBI.jpg
1q0w: Solution structure of Vps27 amino-terminal UIM-ubiquitin complex
PDB 1q5w EBI.jpg
1q5w: Ubiquitin Recognition by Npl4 Zinc-Fingers
PDB 1s1q EBI.jpg
1s1q: TSG101(UEV) domain in complex with Ubiquitin
PDB 1sif EBI.jpg
1sif: Crystal structure of a multiple hydrophobic core mutant of ubiquitin
PDB 1tbe EBI.jpg
1tbe: STRUCTURE OF TETRAUBIQUITIN SHOWS HOW MULTIUBIQUITIN CHAINS CAN BE FORMED
PDB 1ubi EBI.jpg
1ubi: SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1
PDB 1ubq EBI.jpg
1ubq: STRUCTURE OF UBIQUITIN REFINED AT 1.8 ANGSTROMS RESOLUTION
PDB 1ud7 EBI.jpg
1ud7: SOLUTION STRUCTURE OF THE DESIGNED HYDROPHOBIC CORE MUTANT OF UBIQUITIN, 1D7
PDB 1uzx EBI.jpg
1uzx: A COMPLEX OF THE VPS23 UEV WITH UBIQUITIN
PDB 1v80 EBI.jpg
1v80: Solution structures of ubiquitin at 30 bar and 3 kbar
PDB 1v81 EBI.jpg
1v81: Solution structures of ubiquitin at 30 bar and 3 kbar
PDB 1wr1 EBI.jpg
1wr1: The complex structure of Dsk2p UBA with ubiquitin
PDB 1wr6 EBI.jpg
1wr6: Crystal structure of GGA3 GAT domain in complex with ubiquitin
PDB 1wrd EBI.jpg
1wrd: Crystal structure of Tom1 GAT domain in complex with ubiquitin
PDB 1xd3 EBI.jpg
1xd3: Crystal structure of UCHL3-UbVME complex
PDB 1xqq EBI.jpg
1xqq: Simultaneous determination of protein structure and dynamics
PDB 1yd8 EBI.jpg
1yd8: COMPLEX OF HUMAN GGA3 GAT DOMAIN AND UBIQUITIN
PDB 1yiw EBI.jpg
1yiw: X-ray Crystal Structure of a Chemically Synthesized Ubiquitin
PDB 1yj1 EBI.jpg
1yj1: X-ray Crystal Structure of a Chemically Synthesized [D-Gln35]Ubiquitin
PDB 1yx5 EBI.jpg
1yx5: Solution Structure of S5a UIM-1/Ubiquitin Complex
PDB 1yx6 EBI.jpg
1yx6: Solution Structure of S5a UIM-2/Ubiquitin Complex
PDB 1zgu EBI.jpg
1zgu: Solution structure of the human Mms2-Ubiquitin complex
PDB 2ayo EBI.jpg
2ayo: Structure of USP14 bound to ubquitin aldehyde
PDB 2bgf EBI.jpg
2bgf: NMR STRUCTURE OF LYS48-LINKED DI-UBIQUITIN USING CHEMICAL SHIFT PERTURBATION DATA TOGETHER WITH RDCS AND 15N-RELAXATION DATA
PDB 2c7m EBI.jpg
2c7m: HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN
PDB 2c7n EBI.jpg
2c7n: HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN
PDB 2d3g EBI.jpg
2d3g: Double sided ubiquitin binding of Hrs-UIM
PDB 2den EBI.jpg
2den: Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin
PDB 2dx5 EBI.jpg
2dx5: The complex structure between the mouse EAP45-GLUE domain and ubiquitin
PDB 2fcm EBI.jpg
2fcm: X-ray Crystal Structure of a Chemically Synthesized [D-Gln35]Ubiquitin with a Cubic Space Group
PDB 2fcn EBI.jpg
2fcn: X-ray Crystal Structure of a Chemically Synthesized [D-Val35]Ubiquitin with a Cubic Space Group
PDB 2fcq EBI.jpg
2fcq: X-ray Crystal Structure of a Chemically Synthesized Ubiquitin with a Cubic Space Group
PDB 2fcs EBI.jpg
2fcs: X-ray Crystal Structure of a Chemically Synthesized [L-Gln35]Ubiquitin with a Cubic Space Group
PDB 2fid EBI.jpg
2fid: Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin
PDB 2fif EBI.jpg
2fif: Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin
PDB 2fuh EBI.jpg
2fuh: Solution Structure of the UbcH5c/Ub Non-covalent Complex
PDB 2g3q EBI.jpg
2g3q: Solution Structure of Ede1 UBA-ubiquitin complex
PDB 2g45 EBI.jpg
2g45: Co-crystal structure of znf ubp domain from the deubiquitinating enzyme isopeptidase T (isot) in complex with ubiquitin
PDB 2gbj EBI.png
2gbj: Crystal Structure of the 9-10 8 Glycine Insertion Mutant of Ubiquitin.
PDB 2gbk EBI.png
2gbk: Crystal Structure of the 9-10 MoaD Insertion Mutant of Ubiquitin
PDB 2gbm EBI.png
2gbm: Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin
PDB 2gbn EBI.png
2gbn: Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin
PDB 2gbr EBI.png
2gbr: Crystal Structure of the 35-36 MoaD Insertion Mutant of Ubiquitin
PDB 2gmi EBI.jpg
2gmi: Mms2/Ubc13~Ubiquitin
PDB 2hd5 EBI.jpg
2hd5: USP2 in complex with ubiquitin
PDB 2hth EBI.jpg
2hth: Structural basis for ubiquitin recognition by the human EAP45/ESCRT-II GLUE domain
PDB 2ibi EBI.jpg
2ibi: Covalent Ubiquitin-USP2 Complex
PDB 2j7q EBI.jpg
2j7q: CRYSTAL STRUCTURE OF THE UBIQUITIN-SPECIFIC PROTEASE ENCODED BY MURINE CYTOMEGALOVIRUS TEGUMENT PROTEIN M48 IN COMPLEX WITH A UBQUITIN-BASED SUICIDE SUBSTRATE
PDB 2nr2 EBI.jpg
2nr2: The MUMO (minimal under-restraining minimal over-restraining) method for the determination of native states ensembles of proteins
PDB 2o6v EBI.jpg
2o6v: Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH
PDB 2oob EBI.jpg
2oob: crystal structure of the UBA domain from Cbl-b ubiquitin ligase in complex with ubiquitin
