Isopenicillin-N N-acyltransferase

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In enzymology, an isopenicillin-N N-acyltransferase (EC is an enzyme that catalyzes the chemical reaction

phenylacetyl-CoA + isopenicillin N + H2O CoA + penicillin G + L-2-aminohexanedioate

The 3 substrates of this enzyme are phenylacetyl-CoA, isopenicillin N, and H2O, whereas its 3 products are CoA, penicillin G, and L-2-aminohexanedioate.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:isopenicillin N N-acyltransferase. Other names in common use include acyl-coenzyme A:isopenicillin N acyltransferase, and isopenicillin N:acyl-CoA: acyltransferase. This enzyme participates in penicillin and cephalosporin biosynthesis.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
  • PubMed Central references for
  • Google Scholar references for
  • Tobin MB, Fleming MD, Skatrud PL, Miller JR (1990). "Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli". J. Bacteriol. 172: 5908&ndash, 14. PMID 2120195.
  • Aplin RT, Baldwin JE, Roach PL, Robinson CV, Schofield CJ (Pt 2). "Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry". Biochem. J. 294: 357&ndash, 63. PMID 8396910. Check date values in: |date= (help)

External links

The CAS registry number for this enzyme class is 54576-90-8.

Gene Ontology (GO) codes