Polynucleotide 5'-hydroxyl-kinase

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In enzymology, a polynucleotide 5'-hydroxyl-kinase (EC is an enzyme that catalyzes the chemical reaction

ATP + 5'-dephospho-DNA ADP + 5'-phospho-DNA

Thus, the two substrates of this enzyme are ATP and 5'-dephospho-DNA, whereas its two products are ADP and 5'-phospho-DNA.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:5'-dephosphopolynucleotide 5'-phosphotransferase. Other names in common use include ATP:5'-dephosphopolynucleotide 5'-phosphatase, PNK, polynucleotide 5'-hydroxyl kinase (phosphorylating), 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, DNA 5'-hydroxyl kinase, DNA kinase, polynucleotide kinase, and polynucleotide 5'-hydroxy-kinase.

Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1LTQ, 1LY1, 1RC8, 1RPZ, 1RRC, 1UJX, 1YJ5, 1YJM, 2BRF, and 2IA5.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
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  • Google Scholar references for
  • Novogrodsky A, Hurwitz J (1966). "The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5'-hydroxyl termini". J. Biol. Chem. 241: 2923&ndash, 32. PMID 4287929.
  • Novogrodsky A, Tal M, Traub A, Hurwitz J (1966). "The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl polynucleotide kinase". J. Biol. Chem. 241: 2933&ndash, 43. PMID 4287930.

External links

The CAS registry number for this enzyme class is 37211-65-7.

Gene Ontology (GO) codes