Phosphatidylinositol diacylglycerol-lyase

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File:1t6m.gif
Phosphatidylinositol-specific phospholipase C, image from OPM database


In enzymology, a phosphatidylinositol diacylglycerol-lyase (EC 4.6.1.13) is an enzyme that catalyzes the chemical reaction

1-phosphatidyl-1D-myo-inositol 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

Hence, this enzyme has one substrate, 1-phosphatidyl-1D-myo-inositol, and two products, 1D-myo-inositol 1,2-cyclic phosphate and 1,2-diacyl-sn-glycerol.

This enzyme belongs to the family of lyases, specifically the class of phosphorus-oxygen lyases. The systematic name of this enzyme class is 1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase (1D-myo-inositol-1,2-cyclic-phosphate-forming). Other names in common use include monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, 1-phosphatidylinositol phosphodiesterase, 1-phosphatidyl-D-myo-inositol inositolphosphohydrolase, (cyclic-phosphate-forming), 1-phosphatidyl-1D-myo-inositol diacylglycerol-lyase, and (1,2-cyclic-phosphate-forming). This enzyme participates in inositol phosphate metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1T6M and 2OR2.

References

  • IUBMB entry for 4.6.1.13
  • BRENDA references for 4.6.1.13 (Recommended.)
  • PubMed references for 4.6.1.13
  • PubMed Central references for 4.6.1.13
  • Google Scholar references for 4.6.1.13
  • Allan D, Michell RH (1974). "Phosphatidylinositol cleavage catalysed by the soluble fraction from lymphocytes. Activity at pH5.5 and pH7.0". Biochem. J. 142: 591&ndash, 7. PMID 4377210.
  • Friedel RO, Brown JD, Durell (1967). "Monophosphatidyl inositol inositolphosphohydrolase in guinea-pig brain". Biochim. Biophys. Acta. 144: 684&ndash, 6. PMID 4294905.
  • Irvine RF (1982). "The enzymology of stimulated inositol lipid turnover". Cell. Calcium. 3: 295&ndash, 309. PMID 6297738.
  • Michell RH, Allan D (1975). "Inositol cyclis phosphate as a product of phosphatidylinositol breakdown by phospholipase C (Bacillus cereus)". FEBS. Lett. 53: 302&ndash, 4. PMID 236918.
  • Low MG, Finean JB (1977). "Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C". Biochem. J. 167: 281&ndash, 4. PMID 588258.
  • Henner DJ, Yang M, Chen E, Hellmiss R, Rodriguez H, Low MG (1988). "Sequence of the Bacillus thuringiensis phosphatidylinositol specific phospholipase C". Nucleic. Acids. Res. 16: 10383. PMID 3194218.

External links

The CAS registry number for this enzyme class is 37288-19-0.

Gene Ontology (GO) codes


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