Mandelonitrile lyase

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In enzymology, a mandelonitrile lyase (EC is an enzyme that catalyzes the chemical reaction

mandelonitrile cyanide + benzaldehyde

Hence, this enzyme has one substrate, mandelonitrile, and two products, cyanide and benzaldehyde.

This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is mandelonitrile benzaldehyde-lyase (cyanide-forming). Other names in common use include hydroxynitrile lyase, (R)-oxynitrilase, oxynitrilase, D-oxynitrilase, D-alpha-hydroxynitrile lyase, and mandelonitrile benzaldehyde-lyase. This enzyme participates in cyanoamino acid metabolism. It has 2 cofactors: flavin, and Flavoprotein.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1JU2.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
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  • BECKER W, BENTHIN U, ESCHENHOF E, PFEIL E (1963). "[On the knowledge of cyanhydrin synthesis. II. Purification and properties of hydroxynitrilase from bitter almonds (Prunus communis Stokes)]". Biochem. Z. 337: 156&ndash, 66. PMID 13970146.
  • Becker W and Pfeil E (1964). "Die Darstellung kristallisierter Oxynitrilase aus bitteren Mandeln (Prunus comm. Stks)". Naturwissenschaften. 51: 193.
  • Gross M, Jacobs GH, Poulton JE (1982). "A rapid and sensitive spectrophotometric assay for prunasin hydrolase activity employing purified mandelonitrile lyase". Anal. Biochem. 119: 25&ndash, 30. PMID 6803611.
  • Xu LL, Singh BK, Conn EE (1986). "Purification and characterization of mandelonitrile lyase from Prunus lyonii". Arch. Biochem. Biophys. 250: 322&ndash, 8. PMID 3777939.
  • Yemm RS, Poulton JE (1986). "Isolation and characterization of multiple forms of mandelonitrile lyase from mature black cherry (Prunus serotina Ehrh.) seeds". Arch. Biochem. Biophys. 247: 440&ndash, 5. PMID 3717954.

External links

The CAS registry number for this enzyme class is 9024-43-5.

Gene Ontology (GO) codes