Holo-(acyl-carrier-protein) synthase

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In enzymology, a holo-[acyl-carrier-protein] synthase (EC 2.7.8.7) is an enzyme that catalyzes the chemical reaction

CoA-[4'-phosphopantetheine] + apo-[acyl-carrier-protein] adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]

Thus, the two substrates of this enzyme are [[CoA-[4'-phosphopantetheine]]] and [[apo-[acyl-carrier-protein]]], whereas its two products are adenosine 3',5'-bisphosphate and [[holo-[acyl-carrier-protein]]].

This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase. Other names in common use include acyl carrier protein holoprotein (holo-ACP) synthetase, holo-ACP synthetase, coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine, transferase, holosynthase, acyl carrier protein synthetase, holo-ACP synthase, PPTase, AcpS, ACPS, acyl carrier protein synthase, P-pant transferase, and CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase. This enzyme participates in pantothenate and coa biosynthesis.

Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1F7L, 1F7T, 1F80, 1FTE, 1FTF, 1FTH, 2JBZ, and 2JCA.

References

  • IUBMB entry for 2.7.8.7
  • BRENDA references for 2.7.8.7 (Recommended.)
  • PubMed references for 2.7.8.7
  • PubMed Central references for 2.7.8.7
  • Google Scholar references for 2.7.8.7
  • Elovson J, Vagelos PR (1968). "Acyl carrier protein. X. Acyl carrier protein synthetase". J. Biol. Chem. 243: 3603&ndash, 11. PMID 4872726.
  • Prescott DJ, Vagelos PR (1972). "Acyl carrier protein". Adv. Enzymol. Relat. Areas. Mol. Biol. 36: 269&ndash, 311. PMID 4561013.
  • Reid R, Khosla C, Walsh CT (1996). "A new enzyme superfamily - the phosphopantetheinyl transferases". Chem. Biol. 3: 923&ndash, 36. PMID 8939709.
  • Walsh CT, Gehring AM, Weinreb PH, Quadri LE, Flugel RS (1997). "Post-translational modification of polyketide and nonribosomal peptide synthases". Curr. Opin. Chem. Biol. 1: 309&ndash, 15. PMID 9667867.
  • Mootz HD, Finking R, Marahiel MA (2001). "4'-phosphopantetheine transfer in primary and secondary metabolism of Bacillus subtilis". J. Biol. Chem. 276: 37289&ndash, 98. PMID 11489886.
  • Joshi AK, Zhang L, Rangan VS, Smith S (2003). "Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity". J. Biol. Chem. 278: 33142&ndash, 9. PMID 12815048.

External links

The CAS registry number for this enzyme class is 37278-30-1.

Gene Ontology (GO) codes


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