GlmS glucosamine-6-phosphate activated ribozyme

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Glucosamine-6-phosphate activated ribozyme is found in the mRNA for GlmS. GlmS is an enzyme that uses fructose-6-phosphate and glutamine to generate glucosamine-6-phosphate. The ribozyme catalyses self cleavage of the glmS mRNA thereby regulating GlmS activity [1]. The GlmS element is only catalytic when bound to glucosamine-6-phosphate. The glucosamine-6-phosphate is a cofactor for the catalytic reaction, directly participating in the chemistry. This is the only known case of a ribozyme using a small-molecule cofactor at present.

Structure

The structure of the GlmS ribozyme has been determined by X-ray crystallography.[2][3]

References

  1. Winkler, WC (2004). "Control of gene expression by a natural metabolite-responsive ribozyme". Nature. 428: 281&ndash, 286. PMID 15029187. Unknown parameter |coauthors= ignored (help)
  2. Klein DJ, Ferré-D'Amaré AR (2006). "Structural basis of glmS ribozyme activation by glucosamine-6-phosphate". Science. 313 (5794): 1752–6. doi:10.1126/science.1129666. PMID 16990543.
  3. Cochrane JC, Lipchock SV, Strobel SA (2007). "Structural investigation of the GlmS ribozyme bound to Its catalytic cofactor". Chem. Biol. 14 (1): 97–105. doi:10.1016/j.chembiol.2006.12.005. PMID 17196404.
  • Jansen, JA (2006). "Backbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozyme". Nat Struct Mol Biol. 13: 517&ndash, 523. PMID 16699515. Unknown parameter |coauthors= ignored (help)
  • Hampel, KJ (2006). "Evidence for preorganization of the glmS ribozyme ligand binding pocket". Biochemistry. 45: 7861&ndash, 7871. PMID 16784238. Unknown parameter |coauthors= ignored (help)

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