DNA-(apurinic or apyrimidinic site) lyase

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In enzymology, a DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18) is an enzyme that catalyzes the chemical reaction

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

This enzyme belongs to the family of lyases, specifically the "catch-all" class of lyases that cleave carbon-oxygen bonds. The systematic name of this enzyme class is DNA-(apurinic or apyrimidinic site) 5'-phosphomonoester-lyase. Other names in common use include AP lyase, AP endonuclease class I, endodeoxyribonuclease (apurinic or apyrimidinic), deoxyribonuclease (apurinic or apyrimidinic), E. coli endonuclease III, phage-T4 UV endonuclease, Micrococcus luteus UV endonuclease, AP site-DNA 5'-phosphomonoester-lyase, and X-ray endonuclease III.

Structural studies

As of late 2007, 23 structures have been solved for this class of enzymes, with PDB accession codes 1BIX, 1CQG, 1CQH, 1DE8, 1DE9, 1DEW, 1E9N, 1HD7, 1N39, 1N3A, 1N3C, 1VYB, 2ABK, 2I5W, 2ISI, 2J63, 2NOB, 2NOE, 2NOF, 2NOH, 2NOI, 2NOL, and 2NOZ.

References

  • IUBMB entry for 4.2.99.18
  • BRENDA references for 4.2.99.18 (Recommended.)
  • PubMed references for 4.2.99.18
  • PubMed Central references for 4.2.99.18
  • Google Scholar references for 4.2.99.18
  • Bailly V, Sente B, Verly WG (1989). "Bacteriophage-T4 and Micrococcus luteus UV endonucleases are not endonucleases but beta-elimination and sometimes beta delta-elimination catalysts". Biochem. J. 259: 751&ndash, 9. PMID 2471512.
  • Bailly V, Verly WG (1987). "Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst". Biochem. J. 242: 565&ndash, 72. PMID 2439070.
  • Bailly V, Verly WG (1989). "AP endonucleases and AP lyases". Nucleic. Acids. Res. 17: 3617&ndash, 8. PMID 2471157.
  • PH (1988). "Mechanism of UV endonuclease-V cleavage of abasic sites in DNA determined by C-13 labeling". J. Am. Chem. Soc. 110: 2690&ndash, 2691.

External links

The CAS registry number for this enzyme class is 61811-29-8.

Gene Ontology (GO) codes

Template:4.2-enzyme-stub


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