DAPI

Jump to: navigation, search
Template:Chembox E number
DAPI
IUPAC name 2-(4-amidinophenyl)-1H -indole-6-carboxamidine
Identifiers
3D model (JSmol)
ECHA InfoCard Lua error in Module:Wikidata at line 879: attempt to index field 'wikibase' (a nil value). Lua error in Module:Wikidata at line 879: attempt to index field 'wikibase' (a nil value).
Properties
C16H15N5
Molar mass 277.324
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

WikiDoc Resources for DAPI

Articles

Most recent articles on DAPI

Most cited articles on DAPI

Review articles on DAPI

Articles on DAPI in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on DAPI

Images of DAPI

Photos of DAPI

Podcasts & MP3s on DAPI

Videos on DAPI

Evidence Based Medicine

Cochrane Collaboration on DAPI

Bandolier on DAPI

TRIP on DAPI

Clinical Trials

Ongoing Trials on DAPI at Clinical Trials.gov

Trial results on DAPI

Clinical Trials on DAPI at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on DAPI

NICE Guidance on DAPI

NHS PRODIGY Guidance

FDA on DAPI

CDC on DAPI

Books

Books on DAPI

News

DAPI in the news

Be alerted to news on DAPI

News trends on DAPI

Commentary

Blogs on DAPI

Definitions

Definitions of DAPI

Patient Resources / Community

Patient resources on DAPI

Discussion groups on DAPI

Patient Handouts on DAPI

Directions to Hospitals Treating DAPI

Risk calculators and risk factors for DAPI

Healthcare Provider Resources

Symptoms of DAPI

Causes & Risk Factors for DAPI

Diagnostic studies for DAPI

Treatment of DAPI

Continuing Medical Education (CME)

CME Programs on DAPI

International

DAPI en Espanol

DAPI en Francais

Business

DAPI in the Marketplace

Patents on DAPI

Experimental / Informatics

List of terms related to DAPI


DAPI or 4',6-diamidino-2-phenylindole is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI will pass through an intact cell membrane, it may be used to stain both live and fixed cells.

For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. (This emission is fairly broad, and appears blue/cyan.)[1] DAPI will also bind to RNA, though it is not as strongly fluorescent. Its emission shifts to around 500 nm when bound to RNA.[2]

DAPI (magenta) bound to the minor groove of DNA (green and blue). From PDB: 1D30​.

DAPI's blue emission is convenient for microscopists who wish to use multiple fluorescent stains in a single sample. There is fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red, but using spectral unmixing or taking images sequentially can get around this.

Apart from labelling cell nuclei, the most popular application of DAPI is in detection of mycoplasma or virus DNA in cell cultures.

Because DAPI readily enters live cells and binds tightly to DNA, it is toxic and mutagenic. Care should be taken in its handling and disposal.

The Hoechst stains are similar to DAPI in that they are also blue-fluorescent DNA stains which are compatible with both live- and fixed-cell applications.


de:DAPI



Linked-in.jpg