In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms.
↑Smith M, Herrell S, Lusher M, Lako L, Simpson C, Wiestner A, Skoda R, Ireland M, Strachan T (1999). "Genomic organisation of the human chordin gene and mutation screening of candidate Cornelia de Lange syndrome genes". Hum. Genet. 105 (1–2): 104–11. doi:10.1007/s004390051070. PMID10480362.
↑Millet C, Lemaire P, Orsetti B, Guglielmi P, François V (August 2001). "The human chordin gene encodes several differentially expressed spliced variants with distinct BMP opposing activities". Mech. Dev. 106 (1–2): 85–96. doi:10.1016/S0925-4773(01)00423-3. PMID11472837.
↑Pappano WN, Scott IC, Clark TG, Eddy RL, Shows TB, Greenspan DS (September 1998). "Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes". Genomics. 52 (2): 236–9. doi:10.1006/geno.1998.5474. PMID9782094.
↑Bachiller D, Klingensmith J, Kemp C, Belo JA, Anderson RM, May SR, McMahon JA, McMahon AP, Harland RM, Rossant J, De Robertis EM (February 2000). "The organizer factors Chordin and Noggin are required for mouse forebrain development". Nature. 403 (6770): 658–61. doi:10.1038/35001072. PMID10688202.
↑Harris WA, Sanes DH, Reh TA (2011). Development of the Nervous System (Third ed.). Boston: Academic Press. p. 15. ISBN0-12-374539-X.