Biotin synthase

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In enzymology, a biotin synthase (EC is an enzyme that catalyzes the chemical reaction

dethiobiotin + sulfur + 2 S-adenosyl-L-methionine biotin + 2 L-methionine + 2 5'-deoxyadenosine

The 3 substrates of this enzyme are dethiobiotin, sulfur, and S-adenosyl-L-methionine, whereas its 3 products are biotin, L-methionine, and 5'-deoxyadenosine.

This enzyme belongs to the family of transferases, specifically the sulfurtransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is dethiobiotin:sulfur sulfurtransferase. This enzyme participates in biotin metabolism. It employs one cofactor, iron-sulfur.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1R30.


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  • Shiuan D, Campbell A (1988). "Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons". Gene. 67: 203&ndash, 11. PMID 2971595.
  • Zhang S, Sanyal I, Bulboaca GH, Rich A, Flint DH (1994). "The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase". Arch. Biochem. Biophys. 309: 29&ndash, 35. PMID 8117110.
  • Trainor DA, Parry RJ and Gitterman A (1980). "Biotin biosynthesis. 2. Stereochemistry of sulfur introduction at C-4 of dethiobiotin". J. Am. Chem. Soc. 102: 1467&ndash, 1468.
  • Lotierzo M, Tse Sum Bui B, Florentin D, Escalettes F, Marquet A (2005). "Biotin synthase mechanism: an overview". Biochem. Soc. Trans. 33: 820&ndash, 3. PMID 16042606.
  • Berkovitch F, Nicolet Y, Wan JT, Jarrett JT, Drennan CL (2004). "Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme". Science. 303: 76&ndash, 9. PMID 14704425.
  • Ugulava NB, Gibney BR, Jarrett JT (2001). "Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions". Biochemistry. 40: 8343&ndash, 51. PMID 11444981.

External links

The CAS registry number for this enzyme class is 80146-93-6.

Gene Ontology (GO) codes