Jump to: navigation, search

WikiDoc Resources for Axenic


Most recent articles on Axenic

Most cited articles on Axenic

Review articles on Axenic

Articles on Axenic in N Eng J Med, Lancet, BMJ


Powerpoint slides on Axenic

Images of Axenic

Photos of Axenic

Podcasts & MP3s on Axenic

Videos on Axenic

Evidence Based Medicine

Cochrane Collaboration on Axenic

Bandolier on Axenic

TRIP on Axenic

Clinical Trials

Ongoing Trials on Axenic at Clinical

Trial results on Axenic

Clinical Trials on Axenic at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on Axenic

NICE Guidance on Axenic


FDA on Axenic

CDC on Axenic


Books on Axenic


Axenic in the news

Be alerted to news on Axenic

News trends on Axenic


Blogs on Axenic


Definitions of Axenic

Patient Resources / Community

Patient resources on Axenic

Discussion groups on Axenic

Patient Handouts on Axenic

Directions to Hospitals Treating Axenic

Risk calculators and risk factors for Axenic

Healthcare Provider Resources

Symptoms of Axenic

Causes & Risk Factors for Axenic

Diagnostic studies for Axenic

Treatment of Axenic

Continuing Medical Education (CME)

CME Programs on Axenic


Axenic en Espanol

Axenic en Francais


Axenic in the Marketplace

Patents on Axenic

Experimental / Informatics

List of terms related to Axenic

In biology, axenic describes a culture of a particular organism that is entirely free of all other "contaminating" organisms. The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms are also possible.[1] Axenic cultures are useful because all of the organisms present within them are identical or share the same gene pool. Consequently they will generally respond in a more uniform and reproducible fashion, simplifying the interpretation of experiments.

Axenic cultures of microorganisms are typically prepared using a dilution series of an existing mixed culture. This culture is successively diluted to the point where subsamples of it contain only a few individual organisms, ideally only a single individual (in the case of an asexual species). These subcultures are allowed to grow until the identity of their constituent organisms can be ascertained. Selection of only those cultures consisting of the desired type of organism produces the axenic culture. Subcultures that originate from a single organism or cell without genetic change are considered clones.

Axenic cultures are usually checked routinely to ensure that they remain axenic. One standard approach with microorganisms is to spread a sample of the culture onto an agar plate, and to incubate this for a fixed period of time. The agar should be an enriched medium that will support the growth of common "contaminating" organisms. Such "contaminating" organisms will grow on the plate during this period, identifying cultures that are no longer axenic.


  1. Thain, M.; Hickman, M. (1994), Dictionary of Biology (9th edition), Penguin Books, London, UK, ISBN 0-14-051288-8