(heparan sulfate)-glucosamine 3-sulfotransferase 1

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In enzymology, a [heparan sulfate]-glucosamine 3-sulfotransferase 1 (EC is an enzyme that catalyzes the chemical reaction

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate

Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and [[[heparan sulfate]-glucosamine]], whereas its two products are adenosine 3',5'-bisphosphate and [[[heparan sulfate]-glucosamine 3-sulfate]].

This enzyme belongs to the family of transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase. Other names in common use include heparin-glucosamine 3-O-sulfotransferase, 3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase, glucosaminyl 3-O-sulfotransferase, heparan sulfate D-glucosaminyl 3-O-sulfotransferase, and isoform/isozyme 1 (3-OST-1, HS3ST1). This enzyme participates in heparan sulfate biosynthesis and glycan structures - biosynthesis 1.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1VKJ and 1ZRH.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
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  • Kusche M, Backstrom G, Riesenfeld J, Petitou M, Choay J, Lindahl U (1988). "Biosynthesis of heparin. O-sulfation of the antithrombin-binding region". J. Biol. Chem. 263: 15474–84. PMID 3139669. 
  • Shworak NW, Fritze LM, Liu J, Butler LD, Rosenberg RD (1996). "Cell-free synthesis of anticoagulant heparan sulfate reveals a limiting converting activity that modifies an excess precursor pool". J. Biol. Chem. 271: 27063–71. PMID 8900197. 
  • Liu J, Shworak NW, Fritze LM, Edelberg JM, Rosenberg RD (1996). "Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase". J. Biol. Chem. 271: 27072–82. PMID 8900198. 
  • Rosenberg RD (1997). "Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase". J. Biol. Chem. 272: 28008–19. PMID 9346953. 
  • Zhang L, Yoshida K, Liu J, Rosenberg RD (1999). "Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma cells". J. Biol. Chem. 274: 5681–91. PMID 10026187. 
  • RD (2001). "The effect of precursor structures on the action of glucosaminyl 3-O-sulfotransferase-1 and the biosynthesis of anticoagulant heparan sulfate". J. Biol. Chem. 276: 28806–13. PMID 11375390. 

External links

The CAS registry number for this enzyme class is 118113-79-4.

Gene Ontology (GO) codes