(S)-2-hydroxy-acid oxidase

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In enzymology, a (S)-2-hydroxy-acid oxidase (EC is an enzyme that catalyzes the chemical reaction

(S)-2-hydroxy acid + O2 2-oxo acid + H2O2

Thus, the two substrates of this enzyme are (S)-2-hydroxy acid and O2, whereas its two products are 2-oxo acid and H2O2.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is (S)-2-hydroxy-acid:oxygen 2-oxidoreductase. Other names in common use include glycolate oxidase, hydroxy-acid oxidase A, hydroxy-acid oxidase B, glycolate oxidase, oxidase, L-2-hydroxy acid, hydroxyacid oxidase A, L-alpha-hydroxy acid oxidase, and L-2-hydroxy acid oxidase. This enzyme participates in glyoxylate and dicarboxylate metabolism. It employs one cofactor, FMN.

Structural studies

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1AL7, 1AL8, 1GYL, 1TB3, and 2NZL.


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  • Blanchard M, Green DE, Nocito-Carroll V and Ratner S (1946). "l-Hydroxy acid oxidase". J. Biol. Chem. 163: 137–144. 
  • FRIGERIO NA, HARBURY HA (1958). "Preparation and some properties of crystalline glycolic acid oxidase of spinach". J. Biol. Chem. 231: 135–57. PMID 13538955. 
  • KUN E, DECHARY JM, PITOT HC (1954). "The oxidation of glycolic acid by a liver enzyme". J. Biol. Chem. 210: 269–80. PMID 13201588. 
  • Nakano M, Danowski TS (1966). "Crystalline mammalian L-amino acid oxidase from rat kidney mitochondria". J. Biol. Chem. 241: 2075–83. PMID 5946631. 
  • Nakano M, Ushijima Y, Saga M, Tsutsumi Y, Asami H (1968). "Aliphatic L-alpha-hydroxyacid oxidase from rat livers: purification and properties". Biochim. Biophys. Acta. 167: 9–22. PMID 5686300. 
  • Phillips DR, Duley JA, Fennell DJ, Holmes RS (1976). "The self-association of L-alpha hydroxyacid oxidase". Biochim. Biophys. Acta. 427: 679–87. PMID 1268224. 
  • Schuman M, Massey V (1971). "Purification and characterization of glycolic acid oxidase from pig liver". Biochim. Biophys. Acta. 227: 500–20. PMID 5569122. 

External links

The CAS registry number for this enzyme class is 9037-63-2.

Gene Ontology (GO) codes